Lazio secured a 1-0 victory over their city rivals on Sunday at the Stadio Olimpico, which lifted them to second in Serie A.
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Mattia Zaccagni scored the only goal, as Maurizio Sarri's troops moved above Inter into second with 52 points, and five points ahead of Roma.
The Derby della Capitale saw Roma star Roger Ibanez sent off in the first half, before Bryan Cristante and Lazio defender Adam Marusic were given their marching orders at full-time.
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That was not all, though, it has now been revealed Mourinho was involved in a fiery set-to with Lazio owner Lotito in the tunnel.
The Portuguese manager, who is serving a two-match touchline ban, but is allowed in the dressing room, reportedly said: What the f*** are you looking at?"
According to Italian newspaper Il Messaggero, Lotito immediately hit back: "I am the president of Lazio and you are a guest. This is my home and I go wherever I like. And you can't be here."
Both were restrained by Roma assistant coach Salvatore Foti and Lazio midfielder Danilo Cataldi.
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The Italian newspaper also revealed Roma defender Gianluca Mancini called Lazio centre-back Alessio Romagnoli a "piece of s***", sparking further wars between the teams.
Roma's defeat now means they sit fifth in Serie A, having previously looked destined to secure a Champions League place for next season.
"When there is so much talking before a match after, you should remain quiet. Roma didn't play the derby, they only came here to provoke. It happens all the time," said Lazio's Luis Alberto post-match.
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"They talk too much and then they feel f*****. We were good. It was the right moment to take a step forward. Now I'll celebrate with my family."
His teammate Alessio Romagnoli also added: "I've been dreaming of a debut like this in a derby. There won't be a third derby this season, right? We know how they play, but we were better than them. It was important to win and the clean sheet makes the victory even more special."
During the week, after Lazio were dumped out of Europe and Roma advanced in the Europa League, Mourinho quipped: "Obviously I'm not happy that a lot of my players from tonight have to play again on Sunday while Lazio are at home smoking a cigarette with Sarri."