An audible section of the Polish supporters booed and whistled as the Three Lions made their pre-match gesture.
While the home team did not join them in taking the knee, they opted to use the moment to point to the RESPECT badge instead.
talkSPORT commentator Jim Proudfoot narrated the moment: "England players taking the knee. Boos from some of the Polish supporters.
"And, as they do, Robert Lewandowksi just pointed to the RESPECT badge that was on the side of the sleeve of his shirt.
"A nice touch, making his point to the Polish supporters."
Poland first started making this gesture ahead of their first qualifier against England at Wembley back in March.
A statement from the Polish FA at the time read: "The Polish Football Association informs that the players of the Polish national team before the match with England at Wembley took a neutral and apolitical position towards the "Black Lives Matter" initiative.
"At the same time, we emphasise that all Polish representatives are jointly and severally opposed to any manifestations of racism and intolerance.
"In its statutory activities and duties, the PZPN has always been guided by openness, observance of the principles of equality, tolerance and equal rights towards all people, regardless of their place of residence, colour of skin, religion and political views.
"To express their objection, the Polish representatives, before the first whistle of the match with England, pointed to the "UEFA RESPECT" inscription on the left sleeve of the jersey, which refers to the campaign conducted by the European federation against racism, xenophobia and intolerance."
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