Zinedine Zidane's side kept their title hopes alive with a 1-0 success at Athletic Bilbao on Sunday evening, during which Nacho struck a 68th-minute winner for Los Blancos.
The two-horse title race between Real and Atletico Madrid is set to go right down to the wire, and Zidane's side must overcome Villarreal and hope that their rivals slip up against Real Valladolid to be crowned champions.
Here, Sports Mole rounds up all of Real Madrid's latest injury and suspension news ahead of the contest.
Toni Kroos
Status: Major doubt
Type of injury: Self-isolation
Possible return date: May 23 (vs. Villarreal)
Real Madrid revealed last week that Toni Kroos was self-isolating after coming into close contact with a COVID-positive individual. The midfielder returned negative tests, but it remains to be seen if he will be cleared to play this weekend.
Lucas Vazquez
Status: Out
Type of injury: Knee
Possible return date: Unknown
Lucas Vazquez - whose Real Madrid deal expires next month - will miss the final game of the season with a knee injury and may have pulled on the white jersey for the final time.
Dani Carvajal
Status: Out
Type of injury: Hamstring
Possible return date: Unknown
Dani Carvajal's absence has also depleted Zidane's options on the right-hand side of defence, and the Spaniard will not return for Real before the start of the 2021-22 season.
Raphael Varane
Status: Major doubt
Type of injury: Abductor
Possible return date: May 23 (vs. Villarreal)
Raphael Varane was not passed fit for the win over Athletic as he continues to nurse an abductor problem, and the Frenchman is fighting to be fit in time for the final day of the campaign.
Sergio Ramos
Status: Out
Type of injury: Hamstring
Possible return date: Unknown
Sergio Ramos's glittering stint with Real appears to be coming to a bitter end, as the soon-to-be free agent is yet to recover from a hamstring issue and has not put pen to paper on a new deal, so he may have played his final game for the club.
Ferland Mendy
Status: Out
Type of injury: Leg
Possible return date: Unknown
Ferland Mendy has been ruled out for the rest of the season with a troublesome leg issue, and he is facing a race against time to be fit for France's Euro 2020 campaign.
REAL MADRID'S SUSPENSION LIST
Real Madrid have no players suspended for the final day of the season.