Los Blancos could not find a way through at Anfield as they drew 0-0 in the second leg, but a 3-1 first-leg win over Liverpool saw them progress to the final four and keep their hopes of a 14th European crown alive.
However, Zinedine Zidane's men head into this game following a disappointing result in La Liga, as they played out a 0-0 draw with Real Betis and missed the chance to go top.
Here, Sports Mole rounds up all of Real Madrid's injury and suspension news ahead of the first leg.
Sergio Ramos
Status: Out
Type of injury: Calf
Possible return date: Unknown
Captain Sergio Ramos may reportedly be ready to return from a calf issue in time to take part in the second leg on May 5, but the centre-back is sure to miss the first leg and is expected to head for the exit door this summer.
Lucas Vazquez
Status: Out
Type of injury: Knee
Possible return date: Unknown
Another player linked with a move away from the club, Lucas Vazquez suffered a serious knee injury during El Clasico earlier this month and will not be ready for Tuesday's first leg.
Federico Valverde
Status: Out
Type of injury: Coronavirus
Possible return date: Unknown
Federico Valverde initially had to self-isolate after coming into close contact with a COVID-positive individual, and the midfielder is now confirmed to have contracted the virus, so he will play no part in the first leg.
Ferland Mendy
Status: Out
Type of injury: Muscle
Possible return date: Unknown
Real Madrid announced last week that left-back Ferland Mendy had picked up a calf problem, and he was not ready to face Betis. The Frenchman is reportedly yet to return to full training, so a comeback here is extremely unlikely.
Toni Kroos
Status: Minor doubt
Type of injury: Muscle
Possible return date: April 27 (vs. Chelsea)
Toni Kroos has now missed Real Madrid's last three games due to muscular discomfort, but his absence against Betis was merely thought to be precautionary and he should return on Tuesday.
REAL MADRID'S SUSPENSION LIST
Real Madrid have no players suspended for the first leg.
Sparta Rotterdam 
Ajax 
Roma 
Parma 
Dundee Utd 
Celtic 
Valencia 
Alaves 
PSV 
Feyenoord 
Bordeaux 
Rennes 
ST Etienne 
Marseille 
Monaco 
Everton 
Chelsea 
Leicester 
Wolverhampton 
Manchester Utd 
Bournemouth 
Real Madrid 
Sevilla 
Cremonese 
Sampdoria 
Tottenham 
Liverpool 
VFL Wolfsburg 
Borussia Dortmund 
Las Palmas 
Espanyol 
Monza 
Juventus 
Lens 
Paris Saint Germain 
Fiorentina 
Udinese 
Benfica 
Estoril 
Inter 
Como 
Manchester City 
Fulham 
Nottingham Forest 
Motherwell 
ST Mirren 
Rangers 
Carrarese 
Arsenal 
Ipswich