Los Blancos will enter the contest off the back of a 2-0 win over Celta Vigo on Saturday, but they remain 11 points behind La Liga leaders Barcelona in the table with only eight games left to play.
Ferland Mendy remains on the sidelines with a calf problem, while David Alaba will miss a second successive match due to the hamstring issue that he suffered against Chelsea in the second leg of their Champions League quarter-final.
There will also be no Dani Ceballos on Tuesday evening, with the Spanish midfielder unavailable for selection due to the booking that he picked up against Celta.
There are two fresh complaints for Real Madrid, with Karim Benzema not being included in the squad due to a minor injury, while Eden Hazard is out due to a problem that he picked up during a training session.
Eduardo Camavinga and Rodrygo had also been doubts for the match, but the pair are included in the squad.
"Benzema and Camavinga are slightly injured, Rodrygo will be available tomorrow," Real Madrid boss Carlo Ancelotti told reporters during his pre-match press conference.
"If he (Benzema) recovers from the knock he will play on Saturday. He's too important for us."
Ancelotti also revealed that Rodrygo was suffering with "a small knee problem" and an illness, but the Brazil international is still in line to feature against Girona.
"He has had a small knee problem which he is managing well. Today it was an intestinal problem. If he is fine tomorrow, he will play.
"He's not a typical striker. He's doing very well and hopefully, he can progress, because I can see he has room for improvement."
Benzema's absence is likely to lead to Rodrygo playing through the middle on Tuesday evening, with Marco Asensio featuring on the right and Vinicius Junior on the left.
Ancelotti also said during his press conference that he was hopeful of having Alaba available for the final of the Copa del Rey against Osasuna on May 6.
The absences on Tuesday have opened the door for both Mario Martin and Sergio Arribas to be included in the squad, with the former looking to add to his one senior appearance, and the latter hoping to make his 14th outing for the first team.
Real Madrid squad vs. Girona:
Courtois, Lunin, Lopes; Carvajal, Militao, Vallejo, Nacho, Odriozola, Vazquez, Rudiger; Kroos, Modric, Camavinga, Valverde, Tchouameni, Martin, Arribas; Asensio, Vinicius, Rodrygo, Mariano