The Gers currently boast a two-goal advantage over the Bundesliga giants following a stunning 4-2 win at the Signal Iduna Park last week.
But Rangers will be wary of the threat Dortmund still pose with Marco Rose's side smashing Borussia Monchengladbach 6-0 at the weekend.
Giovanni van Bronckhorst's men meanwhile followed up their famous victory at the Westfalenstadion by drawing 1-1 to Dundee United.
Rangers v Borussia Dortmund: talkSPORT commentary
This Europa League playoff clash will take place on Thursday, February 24.
talkSPORT 2 will have live and exclusive coverage of the second-leg at Ibrox which will kick off at 8pm.
Commentary will then come from Andy Bargh and former Rangers midfielder Charlie Adam.
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Rangers v Borussia Dortmund: Team news
The hosts have been dealt a blow following the news that Aaron Ramsey has not recovered from a muscle problem in time to feature.
But Leon Balogun is fit to rejoin the squad, with Rangers' only other absentee being Ianis Hagi who is out for the rest of the season.
Dortmund meanwhile have even bigger problems with star striker Erling Haaland not part of the German's travelling squad to Scotland.
The Norwegian is still struggling with a groin issue and joins Manuel Akanji (calf), Dan-Axel Zagadou (thigh), Mateu Morey and Marcel Schmelzer (both knee) on the sidelines for the visitors.
Rangers v Borussia Dortmund: What has been said?
Van Bronckhorst: "We had a great performance last week and we know it is only halfway. Our focus is to play as strong as possible and aim to win the match.
"We played to our strengths and pressed in the right moments. It will be a very exciting game and one you want to play.
"To begin the game and sit deep, I don't think that's in our system.
"With the backing of our fans tomorrow we want another beautiful European night at Ibrox as we aim to get into the next round."