Pompey are currently sitting top of the League One table, while Peterborough head into the weekend in fourth position.
Match preview
Peterborough will carry significant momentum into the weekend after putting together a six-game winning run across all competitions.
In Wednesday's outing, the Posh clinched a 3-1 victory over Stevenage courtesy of goals from Harrison Burrows, Jadel Katongo and Kwame Poku.
Having racked up 71 points from 37 matches, Peterborough are sitting in fourth position and four points adrift of second-placed Derby County, who have played a game more.
The Posh will look to continue their promotion push in Saturday's clash against the league leaders, and while Portsmouth may boast the best defensive record, Peterborough are currently the league's highest scorers with 74 goals.
The hosts have fared well in recent head-to-head home games, having beaten Portsmouth in each of their last four competitive meetings at the Weston Homes Stadium.
Like their hosts, Portsmouth will also head into Saturday's contest in a confident mood after wining eight and drawing three of their last 11 matches since suffering their most recent defeat against Leyton Orient in January.
John Mousinho's side recorded a narrow 2-1 victory in Tuesday's home game meeting with Burton thanks to a brace from Australia international Kusini Yengi.
As a result of the win, Portsmouth remain five points clear of second-placed Derby and six points better off than Bolton Wanderers in third position.
With Derby and Bolton set to face off at the weekend, Pompey will look to move a step closer to promotion with a win on Saturday, although they will be mindful of the fact they have drawn three of their last four away matches.
However, they have proven to be a tough team to break down on their travels, having kept clean sheets in five of their previous six away trips.
Peterborough United League One form:
L
W
W
W
W
W
Peterborough United form (all competitions):
W
W
W
W
W
W
Portsmouth League One form:
W
W
D
W
D
W
Team News
Peterborough are still unable to call upon the injured trio of Will Blackmore, Jacob Wakeling and Jeando Fuchs.
Ryan De Havilland is likely to replace Hector Kyprianou, who is set to serve a two-match suspension after receiving his 10th yellow card of the League One season.
Poku will be looking to continue his goalscoring run, having found the net in three of Peterborough's last four matches.
As for the visitors, Conor Ogilvie, Anthony Scully and Callum Lang have all recently been added to a length injury list.
The visitors are still expected to be without Regan Poole, Tom McIntyre, Ben Stevenson, Tom Lowery, Joe Morrell, Alex Robertson, Terry Devlin and Tino Anjorin.
Left-back Jack Sparkes is expected to return to the matchday squad after he missed Tuesday's win due to a minor injury issue.
Peterborough United possible starting lineup:
Steer; Katongo, Knight, Edwards, Burrows; Kyprianou, Collins; Poku, Randall, Mason-Clark; Jones
Portsmouth possible starting lineup:
Norris; Rafferty, Raggett, Shaughnessy, Sparkes; Pack, Moxon; Kamara, Peart-Harris, Lane; Yengi
Dundee Utd 
Celtic 
Valencia 
Alaves 
PSV 
Feyenoord 
Bordeaux 
Rennes 
ST Etienne 
Marseille 
Monaco 
Everton 
Chelsea 
Leicester 
Wolverhampton 
Manchester Utd 
Bournemouth 
Real Madrid 
Sevilla 
Cremonese 
Sampdoria 
Tottenham 
Liverpool 
VFL Wolfsburg 
Borussia Dortmund 
Las Palmas 
Espanyol 
Monza 
Juventus 
Lens 
Paris Saint Germain 
Fiorentina 
Udinese 
Benfica 
Estoril 
Inter 
Como 
Manchester City 
Fulham 
Nottingham Forest 
Motherwell 
ST Mirren 
Rangers 
Carrarese 
Arsenal 
Ipswich 
Cagliari 
Lazio 
Atalanta