The hosts have claimed maximum points from their opening two qualifiers, while the visitors remain unbeaten heading into the clash at Abuja National Stadium.
Match preview
Off the back of a 2-1 victory against Sierra Leone to get their qualification journey underway, Nigeria strolled to an impressive 10-0 victory against Sao Tome and Principe on June 13.
Victor Osimhen was the star of the show for Jose Peseiro's men as he netted four times and set up further strikes for Moses Simon and Terem Moffi, who were joined on the scoresheet by Peter Etebo, Ademola Lookman and Emmanuel Dennis.
However, the Super Eagles have been beaten in each of their subsequent three outings which luckily for them, have all been largely inconsequential friendlies.
They will now seek to return to winning ways against an opposing outfit who suffered a 2-0 defeat in the only previous edition of this fixture back in the 2022 AFCON.
Currently unbeaten on home turf since 2021, Friday's hosts will be looking put on a dominant display in front of what is set to be an expectant home crowd.
Guinea-Bissau were denied the opportunity to maintain their perfect AFCON qualifying record following a 2-2 stalemate against Sierra Leone at the General Lansana Conte Stadium in June.
After storming to a two-goal lead courtesy of Jorginho's brace, Baciro Cande's looked to be on course for a huge win before late goals from Augustus Kargbo and Musa Kamara forced a share of the spoils.
With four points from their opening two matches, Djurtus sit second in Group A, two points adrift of this weekend's opponents ahead of the contest in the Nigerian capital.
Having fared poorly on their recent travels, Friday's visitors are faced with yet another daunting task on the road where they have won just one of their last nine matches across all competitions.
Nigeria Africa Cup of Nations Qualifying form:
W
W
Nigeria form (all competitions):
L
W
W
L
L
L
Guinea-Bissau Africa Cup of Nations Qualifying form:
W
D
Guinea-Bissau form (all competitions):
W
W
W
D
L
D
Team News
Following his two-goal haul against Sierra Leone, Jorginho is now up to five international strikes for Guinea-Bissau and continues to move up the all-time scoring charts.
Having marked his first outing for the away side with a goal, 25-year-old Jardel will be pushing for a place in the starting lineup for this one.
Kingsley Aniagboso and Victor Sochima have both been handed their maiden call-up to Nigerian national team but neither is expected to displace Francis Uzoho between the sticks.
Arguably the hottest property in European football right now, Victor Osimhen is set to lead the hosts' attack in a two-man strike partnership alongside Terem Moffi.
Osimhen, who has now netted 15 times for his country, currently sits ninth in the all-time scoring charts while also boasting the best goal-to-game ratio of anybody in the top 10.
Nigeria possible starting lineup:
Uzoho; Sanusi, Bassey, Ajayi, Osayi-Samuel; Iwobi, Ndidi, Aribo, Lookman; Osimhen, Moffi
Guinea-Bissau possible starting lineup:
Mendes; Mane, Nanu, Cande, Nogueira; Cassama, Semedo, Rodrigues; Piqueti, Jorginho, Gano