Louis van Gaal's side recorded a 2-0 victory over Senegal on Monday, while Ecuador were also 2-0 winners in their Group A opener, beating the tournament hosts Qatar on Sunday.
Match preview
Netherlands had to be patient against Senegal on Monday, as it did appear that the points were going to be shared in their opening match, with the two teams level at 0-0 heading towards the final whistle.
Cody Gakpo sent Holland ahead in the 84th minute of the contest, though, before Davy Klaassen secured the points in the ninth minute of added time at the end of the 90, with Van Gaal's side making the perfect start to their Group A campaign.
Netherlands will face Ecuador on Friday before rounding off their group stage against the hosts Qatar next Tuesday, and it will be fascinating to see what this group of players can achieve in the tournament, as Holland have impressed in their last two World Cups.
The national team, who have never won the World Cup, were absent from the finals back in 2018, but they reached the final of the 2010 competition before finishing third in 2014.
Should Van Gaal's side manage to win Group A, they would take on the runner-up from Group B in the last-16 stage of the competition, which could be England, Wales, the USA or Iran.
Netherlands will not be looking too far ahead at this stage, though, especially ahead of a contest with a talented Ecuador outfit, who proved to be too strong for the hosts in the opening match on Sunday.
Enner Valencia scored both goals against Qatar, with the former West Ham United forward netting from the penalty spot in the 16th minute before making it 2-0 in the 31st minute.
Ecuador now have their toughest two games in the section, facing Netherlands on Friday before taking on Senegal next Tuesday, and the latter will be desperate to bounce back from their loss to Holland.
La Tricolor did not qualify for the 2018 World Cup, but they were present at the 2014 competition, being eliminated in the group stage, while their best-ever performance came in 2006, with the tournament seeing them advance to the last-16 stage before losing to England.
There had been some doubt over Ecuador's spot in Qatar, with Chile and Peru lodging appeals due to the involvement of Byron Castillo during the qualification fixtures, but the Court of Arbitration for Sport agreed with FIFA in saying that the 23-year-old was able to represent the national side.
Ecuador secured their spot in the finals by finishing fourth in their South American qualification campaign, picking up 26 points from 18 matches.
La Tri only lost one of their last seven matches in the qualification phase, with the run including 1-1 draws with Brazil and Argentina, which is an indication of their credentials, and they will certainly be confident of putting another victory on the board in this match.
Netherlands World Cup form:
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Netherlands form (all competitions):
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D
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Ecuador World Cup form:
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Ecuador form (all competitions):
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Team News
Memphis Depay was not deemed fit enough to start for Netherlands against Senegal due to his injury problems for Barcelona this season, but he played the final 30 minutes of the contest.
The attacker, who has 42 goals at international level, could now come in for a start here, replacing Vincent Janssen, with Gakpo and Steven Bergwijn also set to feature in the final third of the field.
There are unlikely to be any changes elsewhere, with a 3-5-2 formation set to include Frenkie de Jong and Steven Berghuis in midfield, and Denzel Dumfries and Daley Blind in the full-back areas.
Marten de Roon was an injury doubt ahead of the contest with Senegal, but the midfielder also featured off the bench last time out, and he is again expected to receive minutes at some stage of the contest.
As for Ecuador, Valencia suffered an injury to his knee during the clash with Qatar, but the 33-year-old is expected to be available to start this match.
Valencia is the national team's all-time leading goalscorer with 37, while he now has five strikes at World Cups, so the medical team will be working to ensure that he is able to feature here.
Head coach Alfaro could make just the one change from the side that started against Qatar, with Jeremy Sarmiento potentially being introduced for Michael Estrada in what would be a change of shape.
Robert Arboleda is pushing for a spot at the back, though, and the 31-year-old will come into the manager's thinking ahead of kickoff on Friday.
Netherlands possible starting lineup:
Noppert; De Ligt, Van Dijk, Ake; Dumfries, F de Jong, Berghuis, Blind; Gakpo; Memphis, Bergwijn
Ecuador possible starting lineup:
Galindez; Preciado, Torres, Hincapie, Estupinan; Plata, Caicedo, Mendez, Ibarra; Sarmiento; Valencia
We say: Netherlands 2-1 Ecuador
This has the potential to be a very, very difficult match for Netherlands, and it would not be a surprise to see Ecuador triumph, or for the points to be shared. Van Gaal's side were impressive against Senegal, though, and we just have a feeling that they will navigate their way to a huge three points here.