Held at home by Roma on Saturday, the Bianconeri have taken five points from three games as they begin their quest to regain the Scudetto after two fallow years, while their visitors are just a point behind after drawing 2-2 with Sassuolo.
Match preview
Needing a fast start after their disjointed showing in a grim goalless draw with Sampdoria five days earlier, Juventus began Saturday's clash with likely top-four rivals Roma in the best possible manner.
Dusan Vlahovic thundered home a ferocious free kick to give Juve the lead after just one minute and 16 seconds - the fastest such strike in Serie A since records started 18 years ago - and the hosts seemed well on their way to victory.
When Manuel Locatelli then fired in a second, under-fire coach Max Allegri would have breathed a deep sigh of relief, but the VAR subsequently ruled the Italy midfielder's strike out for a Vlahovic handball. After Tammy Abraham then turned in a second-half equaliser, the game was destined for a 1-1 draw.
With big-name summer signings Angel Di Maria and Paul Pogba not available to inspire them due to injury, the Bianconeri have been unimpressive since the opening day, but with some tough fixtures ahead, they must surely target Wednesday's home match for maximum points.
Facing Fiorentina in Serie A at the weekend, and then visiting Paris Saint-Germain in the Champions League, Juve first need to step up their efforts at the Allianz Stadium.
Allegri led his side to 35 points on home soil last term - including three taken from Spezia in March - but that was only Serie A's seventh-best total; not sufficient for a title challenge by any stretch of the imagination.
Tackling their third top-flight campaign under a third different head coach, Spezia have made their brightest start to a Serie A season so far - with four points from their opening three games.
Appointed as Thiago Motta's successor in the summer, Luca Gotti has steered his new side to that tally by way of an opening day win over Empoli and Saturday's 2-2 draw with Sassuolo.
Thumped 3-0 by Inter in-between, the Aquilotti certainly did not benefit from a kind fixture list when the schedule was published for the current campaign, as not only have they had to travel to San Siro and now visit Turin, they must play Napoli and Lazio in their next two road games.
Therefore, making the most of home advantage has been paramount, so after battling back from behind to lead Sassuolo at the interval, Gotti will have been frustrated that his team failed to secure another victory at Stadio Alberto Picco by conceding early in the second half.
Like Juventus, Spezia posted identical win-draw-loss records at home and away last term, but having suffered defeat in all four meetings with their fellow Bianconeri since promotion, matching them at the Allianz Stadium will be a tough task.
Juventus Serie A form:
Juventus form (all competitions):
Spezia Serie A form:
Spezia form (all competitions):
Team News
After promoting young midfielder Fabio Miretti to the starting lineup against Roma, Max Allegri may make some further changes in midweek, as his squad is already diminished by injury and there are bigger games ahead.
While first-choice goalkeeper Wojciech Szczesny returned to the team on Saturday, and new signing Arkadiusz Milik featured from the bench, Juventus were without captain Leonardo Bonucci, plus longer-term absentees Paul Pogba and Federico Chiesa.
Bonucci may be back in time for Spezia's visit, but otherwise the versatile Danilo may deputise again, or Daniele Rugani could line up in central defence.
Meanwhile, Spezia travel to Turin without veteran midfielder Albin Ekdal, who was sent off late on against Sassuolo and must now serve a suspension.
Wing-backs Salva Ferrer and Kelvin Amian both seem set to miss out through injury, and French forward Janis Antiste remains a doubt.
After scoring his second goal of the season from the spot on Saturday, M'Bala Nzola should once more start up front, with Daniele Verde likely to be promoted from the bench to support him after recovering from injury.
Juventus possible starting lineup:
Szczesny; Danilo, Rugani, Bremer, Sandro; Miretti, Locatelli, McKennie; Cuadrado, Vlahovic, Kostic
Spezia possible starting lineup:
Dragowski; Hristov, Kiwior, Nikolaou; Gyasi, Sala, Bourabia, Bastoni, Reca; Verde, Nzola