The hosts secured their biggest ever European victory in their opening group game last week, while the visitors were forced to settle for a score draw.
Match preview
Istanbul Basaksehir have put together an impressive 20-game unbeaten run across all competitions, dating back to March last season, and they have won each of their last five matches this term.
Emre Belozoglu's men secured a comfortable 4-0 away victory over Hearts in their opening Europa Conference League game last week, with Hasan Ali Kaldirim, Youssouf Ndayishimiye, Stefano Okaka and Berkay Ozcan all on the scoresheet in Edinburgh.
The Grey Owls have since claimed a slender 1-0 Super Lig victory away at city rivals Besiktas, with a strike from Bertrand Traore helping them move to within one point of league leaders Konyaspor with a game in hand.
Spirits are high within the Basaksehir camp and they will be keen to claim another three points against Fiorentina as they bid to qualify for the knockout rounds in European competition for only the second time in their history.
Basaksehir have only faced Italian opposition twice before in Europe, but they were beaten 3-0 at home and 4-0 away against Roma in the 2019-20 Europa League group stage.
In stark contrast, Fiorentina have endured a frustrating start to the new campaign and have failed to win any of their last seven games across all competitions.
La Viola played out a 1-1 draw at home to Latvian minnows Rigas Futbola Skola in their opening Europa Conference League group game last week, with Antonin Barak's opener midway through the second half cancelled out by a strike from Andrej Ilic in the 74th minute.
Since then, Fiorentina suffered a 2-1 Serie A defeat away against Bologna last weekend. All three goals were scored within eight second-half minutes, with the hosts coming from behind to secure maximum points courtesy of strikes from Musa Barrow and Marko Arnautovic.
La Viola have now failed to win any of their last eight competitive away matches and head coach Vincenzo Italiano is under pressure to turn the club's fortunes around.
Competing in European competition for the first time in six years, Fiorentina have won all six of their previous meetings against Turkish opposition, conceding just one goal in the process, which bodes well for the Italian outfit heading into Thursday's contest.
Istanbul Basaksehir Europa Conference League form:
Istanbul Basaksehir form (all competitions):
Fiorentina Europa Conference League form:
Fiorentina form (all competitions):
Team News
Istanbul Basaksehir playmaker Mesut Ozil has missed the last three games due to injury, but he has been spotted in training this week and could be in contention to return to the matchday squad.
Goalkeeper Muhammed Sengezer was handed a start between the sticks against Hearts, but Volkan Babacan returned to the first XI against Besiktas and he could retain his place on Thursday.
Danijel Aleksic, Serdar Gurler and Stefano Okaka could all return to the starting lineup after they began on the substitutes' bench last weekend.
As for Fiorentina, Nikola Milenkovic (thigh), Gaetano Castrovilli (knee) and Dodo (calf) are all ruled out with injuries, while Alfred Duncan is doubtful and will be assessed ahead of kickoff.
Igor Julio was sent off in the Serie A defeat to Bologna, but he is available to play on Thursday and will likely start at centre-back alongside Lucas Martinez Quarta, who scored in the same game last weekend.
Should Italiano opt to freshen up his forward line, Arthur Cabral and Jonathan Ikone could be recalled at the expense of Luka Jovic and Riccardo Sottil respectively.
Istanbul Basaksehir possible starting lineup:
Babacan; Sahiner, Duarte, Ndayishimiye, Kaldirim; Tekdemir, Biglia, Ozcan; Traore, Okaka, Gurler
Fiorentina possible starting lineup:
Terracciano; Sorokins, Martinez Quarta, Igor, Biraghi; Bonaventura, Amrabat, Barak; Kouame, Cabral, Ikone
We say: Istanbul Basaksehir 2-1 Fiorentina
The two teams head into this encounter in contrasting form, with Istanbul Basaksehir going from strength to strength so far this season.
Fiorentina 
Udinese 
Benfica 
Estoril 
Inter 
Como 
Manchester City 
Everton 
Chelsea 
Fulham 
Nottingham Forest 
Tottenham 
Celtic 
Motherwell 
Wolverhampton 
Manchester Utd 
ST Mirren 
Rangers 
Sampdoria 
Carrarese 
Liverpool 
Leicester 
Arsenal 
Ipswich 
Cagliari 
Lazio 
Atalanta 
FC Porto 
Boavista 
Torino 
Napoli 
Venezia 
ST Johnstone 
Juventus 
West Ham 
Pisa 
AC Milan 
Roma 
Sporting CP 
Newcastle 
Brentford