Bidding to face either West Ham United or AZ Alkmaar in the final, Fiorentina and Basel will both be eager to notch a first-leg advantage in Tuscany.
Match preview
Fiorentina booked their place in the last four of the Conference League with a dramatic 6-4 aggregate triumph over Polish club Lech Poznan, with La Viola reaching a first European semi-final since 2014-15.
After claiming an impressive 4-1 victory in Poland, Fiorentina found themselves 3-0 down on home soil in the second leg, but late goals from Riccardo Sottil and Gaetano Castrovilli sealed the deal for Vincenzo Italiano's side.
Fiorentina have lost just two of their 12 Conference League clashes this season, notching nine consecutive victories between October and April while scoring at least three goals on seven occasions during that period.
Given that La Viola sit eighth in the Serie A table - 12 points outside the top six - Italiano and his players will view the Conference League as their best chance of securing European football for next season.
Following a 1-0 defeat to newly-crowned Serie A champions Napoli on Sunday, Fiorentina have won just one of their last seven matches in all competitions, so the Tuscany outfit will be hoping to rediscover their best form on Thursday.
Just like Fiorentina, Basel experienced a wild ride en route to the Conference League semi-finals, snatching a 4-3 aggregate victory over French side Nice to book their place in the last four.
Braces from Zeki Amdouni and Terem Moffi ensured that Basel and Nice drew the first leg 2-2, while late goals from Jean-Kevin Augustin and Kasim Nuhu saw the Swiss outfit come from behind to win the second leg 2-1.
It is worth remembering that Basel had to come through three qualifying rounds to make the Conference League group stage, dispatching Crusaders, Brondby and CSKA Sofia before losing just one of their six group games.
That said, FCB have struggled to replicate their European form on the domestic stage this season, with Sunday's 2-0 defeat to FC Zurich leaving Heiko Vogel's side fifth in the Swiss Super League table.
Basel's inconsistency is underlined by a record of three wins, three draws and three losses across their last nine outings in all competitions, making it difficult to predict how Vogel's men will fare when they visit Florence.
Fiorentina Europa Conference League form:
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Fiorentina form (all competitions):
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Basel Europa Conference League form:
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Basel form (all competitions):
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Team News
Fiorentina look set to be without key striker Arthur Cabral for the first leg, as the Brazilian missed Sunday's defeat to Napoli through injury.
Having scored 15 goals this season - including six in his last seven Conference League appearances - Cabral will be missed by the hosts, with Luka Jovic expected to lead the line on Thursday.
La Viola also have veteran goalkeeper Salvatore Sirigu on the treatment table, meaning that Pietro Terracciano will continue between the sticks.
Morocco international Sofyan Amrabat will anchor the midfield alongside Alfred Duncan, allowing Giacomo Bonaventura to push forward in support of Jovic.
As for Basel, Spanish defender Arnau Comas remains sidelined with a thigh injury, while experienced midfielder Fabian Frei will miss Thursday's clash due to a back injury.
There are also question marks over the fitness of Andy Pelmard and Riccardo Calafiori, who both missed Sunday's defeat to FC Zurich.
Having both scored five Conference League goals this term, Zeki Amdouni and Andi Zeqiri will continue in attack, with Taulant Xhaka and Wouter Burger operating in central midfield.
Fiorentina possible starting lineup:
Terracciano; Dodo, Milenkovic, Igor, Terzic; Amrabat, Duncan; Gonzalez, Bonaventura, Sottil; Jovic
Basel possible starting lineup:
Hitz; Lang, Kasim, Lopez; Ndoye, Xhaka, Burger, Millar; Males, Amdouni; Zeqiri