The Peruvian side are currently rock-bottom of Group F after picking up one point from their four matches, while Fortaleza sit in third place with four points to their name.
Match preview
With six points separating them from the top two, a place in the knockout stages of the Copa Libertadores may be out of Alianza Lima's reach.
However, they will be targeting third position in the group, which would guarantee them a spot in the knockout rounds of the Copa Sudamericana.
After losing their opening three matches of the group stage, Alianza Lima finally got a point on the board in their fourth outing as they played out a 1-1 draw against Colo Colo.
A goal from Wilmer Aguirre cancelled out Juan Martin Lucero's opener, ensuring the Peruvian champions remained three points behind Fortaleza.
The draw against Colo Colo features in a four-game unbeaten run that Alianza Lima are currently enjoying, and they have a chance to clinch a third consecutive victory in all competitions on Wednesday.
Fortaleza, meanwhile, have struggled for wins in recent weeks, with their last five matches in all competitions yielding just one victory.
Although the Brazilian side currently sit bottom of the Campeonato Brasileiro Serie A table, they are still in contention for a top two finish in their Copa Libertadores group.
After losing their opening two group matches, Fortaleza have collected four points from their last two Copa Libertadores games, leaving them three points adrift of Colo Colo who occupy second spot.
However, reaching the knockout stages could prove tricky, with their final two group games taking place away from home.
If they are unable to clinch a place in the last 16 of the Copa Libertadores, Fortaleza will be aiming to retain third spot, and a victory on Wednesday would at the very least guarantee them a place in the Copa Sudamericana knockout stages.
Alianza Lima Copa Libertadores form:
Alianza Lima form (all competitions):
Fortaleza Copa Libertadores form:
Fortaleza form (all competitions):
Team News
Jefferson Farfan will miss out for the hosts due to a knee injury, while Aldair Rodriguez is suspended for Wednesday's contest.
Arley Rodriguez came into the starting lineup at the weekend for the league encounter against Cesar Vallejo, and the 29-year-old could feature once again, with Alianza Lima set to name an unchanged lineup.
Hernan Barcos will be aiming to score in a third consecutive match, with the striker set to partner Aguirre in the frontline.
As for the visitors, they have doubts about the availability of goalkeeper Max Walef, who has missed Fortaleza's last two matches due to an eye problem.
If Walef cannot feature then Marcelo Boeck will continue to deputise in goal for the Brazilian side.
Silvio Romero is suspended after receiving a red card on matchday four, which could present Renato Kayser with a starting place.
Alianza Lima possible starting lineup:
Campos; Vilchez, Ramos, Portales, Rojas; Rodriguez, Concha, Ballon, Lavandeira; Aguirre, Barcos
Fortaleza possible starting lineup:
Boeck; Tinga, Benevenuto, Titi; Pikachu, Hercules, Felipe, Crispim; Moises, Romero