The Oranje enjoyed an unbeaten group-stage campaign as they picked up two wins and one draw in their three matches to finish top of Group A with a one-point lead over runners-up Senegal.
Louis van Gaal's men are now unbeaten in each of their last 18 matches across all competitions, picking up 13 wins and five draws since a 2-0 defeat at the hands of Czech Republic in June's Euros. last-16 clash.
A first-half strike from Chelsea forward Christian Pulisic was enough to fire USA to a third consecutive knockout-stage appearance as the Stars and Stripes secured a 1-0 victory over Iran in last Tuesday's group finale.
Prior to that, Gregg Berhalter's men were involved in a share of the spoils in their opening two games in Group B, where they played out a 1-1 draw with Wales on November 21 before holding out for a goalless draw versus England four days later.
Here, Sports Mole looks at how the squads of Netherlands and USA stack up against each other ahead of Saturday's highly-anticipated last-16 matchup.
NETHERLANDS
Most valuable player:
Matthijs de Ligt (£60.8m)
Overall squad value:
£510.1m
Most capped player:
Daley Blind (97)
Leading goalscorer:
Memphis Depay (42)
Total caps:
728
Total goals:
110
Oldest player:
Remko Pasveer (39)
Youngest player:
Xavi Simons (19)
Average age:
26.6
SQUAD IN FULL
Goalkeepers: Justin Bijlow (Feyenoord), Andries Noppert (Heerenveen), Remko Pasveer (Ajax)
Defenders: Virgil van Dijk (Liverpool), Nathan Ake (Manchester City), Daley Blind (Ajax), Jurrien Timber (Ajax), Denzel Dumfries (Inter Milan), Stefan de Vrij (Inter Milan), Matthijs de Ligt (Bayern Munich), Tyrell Malacia (Manchester United), Jeremie Frimpong (Bayer Leverkusen)
Midfielders: Frenkie de Jong (Barcelona), Steven Berghuis (Ajax), Davy Klaassen (Ajax), Teun Koopmeiners (Atalanta BC), Marten de Roon (Atalanta BC), Kenneth Taylor (Ajax), Xavi Simons (PSV Eindhoven)
Forwards: Cody Gakpo (PSV Eindhoven), Memphis Depay (Barcelona), Steven Bergwijn (Ajax), Vincent Janssen (Antwerp), Luuk de Jong (PSV Eindhoven), Noa Lang (Club Brugge), Wout Weghorst (Besiktas)
USA
Most valuable player:
Christian Pulisic (£33m)
Overall squad value:
£241m
Most capped player:
DeAndre Yedlin (75)
Leading goalscorer:
Christian Pulisic (22)
Total caps:
701
Total goals:
83
Oldest player:
Tim Ream (35)
Youngest player:
Yunus Musah (19)
Average age:
25.2
SQUAD IN FULL
Goalkeepers: Matt Turner (Arsenal), Sean Johnson (New York City FC), Ethan Horvath (Luton Town)
Defenders: DeAndre Yedlin (Inter Miami), Tim Ream (Fulham), Walker Zimmerman (Nashville SC), Aaron Long (New York Red Bulls), Antonee Robinson (Fulham), Sergino Dest (AC Milan), Shaq Moore (Nashville SC), Cameron Carter-Vickers (Celtic), Joe Scally (Borussia Monchengladbach)
Midfielders: Kellyn Acosta (Los Angeles FC), Weston McKennie (Juventus), Tyler Adams (Leeds United), Cristian Roldan (Seattle Sounders), Brenden Aaronson (Leeds United), Yunus Musah (Valencia), Luca de la Torre (Celta Vigo)
Forwards: Christian Pulisic (Chelsea), Jordan Morris (Seattle Sounders), Tim Weah (Lille), Josh Sargent (Norwich City), Jesus Ferreira (Dallas), Giovanni Reyna (Borussia Dortmund), Haji Wright (Antalyaspor)
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