The Red Devils signed six new players during the summer transfer window, with Tyrell Malacia, Christian Eriksen, Lisandro Martinez, Casemiro, Antony and Martin Dubravka all arriving at the club.
The new signings are all in the squad, while there are spots for the likes of Nathan Bishop, Brandon Williams and Axel Tuanzebe despite the trio being out of favour under new head coach Erik ten Hag.
Anthony Elanga and Alejandro Garnacho are notable absentees, but the duo do not need to be included in the 25-man squad, as they are both under the age of 21.
As a result, other youth prospects, including Charlie McNeill, who made his first-team debut against Real Sociedad in the Europa League last week, can be used during the season.
Williams was left out of Man United's Europa League squad, with the defender down the pecking order, but he has been included in the squad for the Premier League.
Jones's absence is arguably the main talking point, with the 30-year-old now not eligible to represent the 20-time English champions in the Premier League.
The Englishman spent almost two years on the sidelines with a knee injury between January 2020 and January 2022, which has seen his career stall.
The defender made five appearances for Man United last season, including four outings in the Premier League, but he is in the final year of his deal at Old Trafford and is expected to leave next June.
Jones has been with the Red Devils since 2011, featuring on 229 occasions in all competitions, winning the Premier League, FA Cup, Community Shield and Europa League during his time at the club.
DC United, in addition to a number of Championship clubs, were linked with Jones over the summer, but the transfer window closed with the Englishman still at Old Trafford.
Cristiano Ronaldo was widely expected to leave the Red Devils over the summer, with the 37-year-old determined to play in the Champions League this season.
However, the Portugal international stayed with Man United beyond the deadline, and he will be at the club until at least the January transfer window.
Ronaldo is yet to get off the mark in terms of goals this season, though, drawing blanks in each of his seven appearances in all competitions.
The Portuguese is likely to again be in the starting XI against Sheriff Tiraspol in the Europa League on Thursday, with Marcus Rashford not in the travelling squad, allegedly due to a slight injury.
Full Premier League squad: Nathan Bishop, David De Gea, Martin Dubravka, Tom Heaton; Diogo Dalot, Victor Lindelof, Harry Maguire, Tyrell Malacia, Lisandro Martinez, Luke Shaw, Axel Tuanzebe, Raphael Varane, Aaron Wan-Bissaka, Brandon Williams; Casemiro, Christian Eriksen, Bruno Fernandes, Fred, Scott McTominay, Donny van de Beek; Antony, Anthony Martial, Marcus Rashford, Cristiano Ronaldo, Jadon Sancho