His 56th minute winner against Espanyol saw him overtake Ladislau Kubala and is now on 195 total goals for the Spanish club.
The Uruguayan had already been climbing the all-time list this season when he overtook Josep Samitier's 184 goals earlier in the campaign.
The 33-year-old has achieved that feat in just 278 matches across six seasons at Barcelona.
He joined the LaLiga club from Liverpool in 2014 for £65million where he quickly formed a formidable partnership with Lionel Messi.
Suarez has won 14 trophies during those six season while also cementing his legacy as one of the finest strikers of his generation.
Barcelona's all-time top scorers
Lionel Messi - 630
Cesar - 232
Luis Suarez - 195
Ladislau Kubala - 194
Josep Samitier - 184
Josep Escolà - 167
Paulino Alcántara - 143
Samuel Eto'o - 130
Rivaldo - 130
Mariano Martin - 128
His best season was in 2015/16 when he netted 59 times in 53 games.
There are now just two people ahead of Suarez in the all-time Barcelona charts. He needs 37 to overtake Cesar, who has 232 for the club.
Messi leads the way with an incredible 630 goals for Barcelona.
Suarez moving up to third in the last has highlighted just how ridiculous the Argentinian has been for the Catalan club.
Messi has more goals than the three highest scorers below him in Cesar, Suarez and Kubala combined.
The 33-year-old forward recently netted his 700th career goal for club and country and shows absolutely no sign of slowing down.
For now, Suarez will not be worrying about climbing that list as there are more important things at stake this season.
The win against Espanyol closed the gap to Real Madrid to just one point but their rivals have a game in hand.
"Our goal is to win each of our three games," Suarez said after the win over Espanyol.
"Then we have to wait for the opponent to slip up. We're only thinking about our own games."
Even if they miss out on the LaLiga title there is still the Champions League to hope for.
Barcelona have the second leg of their last 16 tie against Napoli still to come with it currently tied at 1-1.
Luis Suarez reached another milestone for Barcelona on Wednesday night as he became the club's third top scorer in history.