The Argentine didn't even make the bench for the Stamford Bridge clash with 20-year-old midfielder Harvey White getting the nod.
And it appears the decision to omit Lo Celso was a tactical one with the player insisting that he feels ready to play.
He posted on Instagram: "Regarding the questions I have received I would like to [sic] clear that I am in 100 per cent good physical condition. I have no problem and I feel very well.
"I wish the best to my team in the derby today."
Conte's team selection will raise questions about Lo Celso's future, with a number of players understood to be potential departures in January.
talkSPORT understands record signing Tanguy Ndombele is keen on a loan switch to Paris Saint-Germain, while Dele Alli could be set to leave on loan too.
However, Spurs are reportedly closing in on the signing of Wolves forward Adama Traore with Conte having a vision to convert him into a wing-back.