After dropping out of the Europa League, Leicester beat Randers 7-2 on aggregate in their Europa Conference League play-off round last month.
Now the Foxes will host Rennes who on topped Group G in Europe's new competition.
The Ligue 1 side claimed four points off Tottenham in the group stages and will be a tough test for Leicester.
Leicester v Rennes: Date and start time
This Europa Conference League last-16 first-leg tie will take place on Thursday, March 10.
It will be held at the King Power Stadium is scheduled for a 8pm kick-off.
This will be the first professional meeting between the clubs.
Leicester v Rennes: TV channel and live stream
This clash will be broadcast live on BT Sport ESPN.
Live coverage starts from 7:30pm and BT Sport customers can live stream this via the app using their mobile, tablet or computer devices.
To tune in to talkSPORT or talkSPORT 2, click HERE for the live stream.
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Leicester v Rennes: Team news
Leicester will be without Jamie Vardy with the forward out again with a knee problem.
James Maddison and James Justin are set to return after injury lay-offs, but Wesley Fofana, who has been nearing a comeback from a broken leg sustained in pre-season, is unavailable after testing positive for coronavirus.
It is thought the game will come too soon for Ricardo Pereira (hamstring), while Jonny Evans, Timothy Castagne and Ryan Bertrand remain sidelined.
Leicester provisional squad: Schmeichel, Ward, Jakupovic, Justin, Soyuncu, Amartey, Vestergaard, Thomas, Barnes, Tielemans, Maddison, Albrighton, Choudhury, Dewsbury-Hall, Ndidi, Soumare, Iheanacho, Perez, Daka, Lookman.
Leicester v Rennes: What has been said?
Rennes boss Bruno Genesio: "It's an interesting competition for us and a good way to assess ourselves against a big European club.
"They are favourites and better equipped than us but we have to go there with a lot of confidence and conviction.
"They are a team with a lot of experience, who played in the Champions League and were champions of England not so long ago. It is one of the best teams in this Conference League.
"We watched them and we saw that they were a very good team with talented players capable of making a difference. We also saw that there were things to exploit.
"We go there with ambition, humility but above all with our strengths."