The Portuguese star hit the deck after a fierce collision with Brentford midfielder Christian Norgaard.
After receiving treatment, Jota tried to get back to his feet before dropping back down to the ground, with a stretcher summoned onto the pitch.
He was then carried from the Gtech Community Stadium pitch and replaced by returning star Mohamed Salah.
This came after another injury blow in the first-half with Curtis Jones forced off after a challenge from Norgaard, while Darwin Nunez was brought off at half-time to the concern of Liverpool fans.
While it's yet to be confirmed if this was due to injury, fans will be waiting with bated breath in the hope that their goalscoring star hasn't also fallen foul to their injury curse.
Liverpool have one eye on their Carabao Cup final clash with Chelsea next weekend.
However with Jota and Jones joining a lengthy injury list, Jurgen Klopp will be cursing his luck ahead of the Wembley showdown.
The German was later seen with his head in his hands as more strong challenges from Brentford flew in.
Jota's injury came after he played a key role in Liverpool taking the lead over Brentford.
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Following a big clearance from Virgil van Dijk, Jota played a wonderful cushioned header into the path of Darwin Nunez who scored a delightful dinked effort to put the Reds 1-0 up at half-time.
Despite the concern around Nunez's withdrawal, Liverpool went 2-0 up early in the second half through Alexis Mac Allister, putting the Reds firmly in control.
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The game was then fully put to bed as Salah found the back of the net on his return to action, while Ivan Toney's consolation goal made it 3-1 to the Merseysiders.
More to follow...