Fati has been out for more than 10 months with a knee injury but will be included in the squad providing that he comes through Saturday morning's training session.
Ousmane Dembele, Sergio Aguero, Pedri, Jordi Alba and Martin Braithwaite are definitely out of the contest, though, while Alejandro Balde remains a doubt due to a back issue.
The home side will also be without key midfielder Frenkie de Jong following his red card against Cadiz on Thursday, meaning that Sergi Roberto could play in the middle of the park alongside Gavi and Sergio Busquets.
Ronald Araujo is likely to retain his spot in the middle of the defence, while head coach Ronald Koeman does not have many options at full-back, so Oscar Mingueza and Sergino Dest should both feature once again.
Memphis Depay is a certain starter in the final third of the field, while Luuk de Jong is expected to get the nod at the tip of the attack once again.
There could be an alteration from the goalless draw against Cadiz on Thursday night, though, with Philippe Coutinho potentially coming in as a replacement for Yusuf Demir.
Barcelona possible starting lineup: Ter Stegen; Mingueza, Araujo, Pique, Dest; Gavi, Busquets, Roberto; Coutinho, L de Jong, Memphis