Head coach Ronald Koeman will again be unable to call upon either Ansu Fati or Philippe Coutinho due to long-term knee problems, while Barca also suffered another injury blow against Atletico.
Indeed, Sergio Busquets was forced off the field against Diego Simeone's side in the first half due to a facial injury, and tests at the hospital revealed that the Spaniard had suffered a broken jaw.
Busquets will therefore be unavailable for this match, meaning that there could be a spot in the middle of the park for Sergi Roberto, who might just be preferred to youngster Ilaix Moriba.
Oscar Mingueza was substituted at the interval against Atletico, and it would not be a surprise to see Ronald Araujo handed a spot in the back three for this match.
Ousmane Dembele is also pushing for a spot in the first XI, but it seems likely that Antoine Griezmann will retain his position in the final third of the field alongside Lionel Messi.
Pedri and Sergino Dest were both substituted in the latter stages of Saturday's clash with Atletico, but both changes were tactical, and the pair should be in the starting side for this match.
Barcelona possible starting lineup: Ter Stegen; Araujo, Pique, Lenglet; Dest, Roberto, De Jong, Pedri, Alba; Griezmann, Messi
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