The Catalan outfit have no injury concerns ahead of the match, while Jordi Alba is available despite his red card in the 1-1 draw with Espanyol in La Liga last time out.
Robert Lewandowski, as it stands, can also feature despite the fact that the Poland international is still facing a three-game suspension, which could rule him out of the next league game with Atletico Madrid.
Ronald Araujo was back on the bench against Espanyol after a long period out, and the Uruguay international is expected to come into the starting side for this match.
Inaki Pena might get the nod between the sticks, while Jules Kounde, Eric Garcia and Hector Bellerin could also come into the back four for the Copa del Rey contest.
Franck Kessie will come into the midfield, while it is expected to be a brand-new front three, with Memphis Depay, Ferran Torres and Ousmane Dembele potentially starting.
Memphis is being linked with a move away from Barcelona this month, but the Netherlands international is expected to be given the opportunity to start this match.
Gavi and Frenkie de Jong could again feature in midfield, with Sergio Busquets potentially being saved for the next league game with Atletico.
Barcelona possible starting lineup: Pena; Araujo, Kounde, E Garcia, Bellerin; Kessie, F de Jong, Gavi; Memphis, F Torres, Dembele