Ousmane Dembele, Sergio Aguero, Pedri, Jordi Alba and Martin Braithwaite will all be absent for this match, while Samuel Umtiti could miss out with the muscular issue that forced him to sit out training on Friday.
Youngster Alejandro Balde made his debut off the bench in the 3-0 defeat to Bayern Munich last week and could now come into the starting side at left-back.
Sergio Roberto is expected to play in midfield due to Pedri's absence, while head coach Ronald Koeman's options in the final third are limited due to the team's injury problems.
Memphis Depay is a certain starter in attack, and the Netherlands international could be joined by Luuk de Jong and Philippe Coutinho, who featured off the bench against Bayern.
Koeman is likely to revert to a back four on Monday, which would allow Sergino Dest to return at right-back, while the likes of Gavi and Yusuf Demir are also in contention for spots in the starting side.
There will still be plenty of experience in the side, though, with Marc-Andre ter Stegen, Gerard Pique and Sergio Busquets all in line to feature.
Barcelona possible starting lineup: Ter Stegen; Dest, Araujo, Pique, Balde; Roberto, Busquets, F de Jong; Coutinho, L de Jong, Depay