The Catalan outfit will enter the contest at Wanda Metropolitano off the back of a 3-1 success over Napoli in the second leg of their last-16 Champions League tie, which secured a 4-2 aggregate win.
Xavi could potentially name the same XI for this contest, with Fermin Lopez and Andreas Christensen again both in line for spots in the midfield three.
Frenkie de Jong, Pedri, Gavi and Alejandro Balde are all currently on the sidelines, while the match is also expected to come too soon for Marcos Alonso and Ferran Torres.
Barcelona have no fresh fitness concerns, though, and there are expected to again be starts for Lamine Yamal, 16, and Pau Cubarsi, 17, with both players in excellent form.
Cubarsi put in a man-of-the-match performance against Napoli, and his form has seen him earn a spot in the senior Spain squad for the March internationals.
Joao Felix, who is on loan from Atletico, scored against his parent club in La Liga earlier this season, but the Portugal international is expected to start on the bench this weekend.
Raphinha is likely to join Yamal and Robert Lewandowski in the final third of the field, while Ronald Araujo will be back in a league XI, having missed the 1-0 win over Mallorca last weekend through suspension.
Barcelona possible starting lineup: Ter Stegen; Kounde, Araujo, Cubarsi, Cancelo; F Lopez, Christensen, Gundogan; Yamal, Lewandowski, Raphinha