Head coach Lionel Scaloni has selected a strong squad for the match in London, and Messi could be joined in the final third of the field by Lautaro Martinez and Angel Di Maria.
Argentina are set to match up with Italy in terms of a 4-3-3 formation, with Rodrigo De Paul, Guido Rodriguez and Giovani Lo Celso potentially featuring as the midfield three.
At the back, the experienced Nicolas Otamendi is likely to be joined by Cristian Romero, with Marcos Acuna and Nahuel Molina lining up in the full-back positions.
Brighton & Hove Albion's Alexis Mac Allister is also in the selection, while Nehuen Perez and Marcos Senesi will be hoping to be given opportunities to make their first appearances for Argentina.
The South American nation have plenty of talent in the final third of the field, with Paulo Dybala, Angel Correa and Joaquin Correa also included.
There is a spot in the squad for on-loan Manchester City forward Julian Alvarez, meanwhile, in addition to Aston Villa's Emiliano Martinez, who is set to start between the sticks.
Argentina possible starting lineup: E Martinez; Molina, Romero, Otamendi, Acuna; De Paul, Rodriguez, Lo Celso; Messi, L Martinez, Di Maria