Kane was forced off with a knock just 33 minutes into Bayern Munich's 1-1 draw with Borussia Dortmund.
Jamie Gittens handed Dortmund the lead at the Signal Iduna Park with a powerful strike following a dazzling run down the left wing.
Matters were made worse for Bayern just moments later as their top scorer Kane went down onto the pitch.
The 31-year-old was then swiftly substituted by manager Vincent Kompany and there were major concerns over his fitness.
And now the German giants have confirmed that he's facing a spell on the sidelines with a thigh injury.
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A Bayern statement read: "Harry Kane suffered a small muscle fibre tear in his right thigh during yesterday's match against Borussia Dortmund. After an examination by our medical department, Kane will be sidelined for the time being."
With no timeframe being given, it's unknown whether Kane is going to miss any league games, but he may sit out of his side's DFB-Pokal clash on Tuesday night.
The new update came after Kompany played down the severity of the injury following the game.
He remarked: "Harry said it's not too bad. Hopefully that's the case.
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"We'll have the tests today and let's see what happen."
Thomas Muller was the man to replace him after the injury, with Bayern heading into half-time of the Bundesliga clash 1-0 down.
Jamal Musiala equalised with five minutes left to play though as he headed in unmarked inside the box to seal a point.
Kane has scored 20 goals in 19 appearances for the club this season but it remains to be seen when he'll return to action.
Most of those have come in the Bundesliga and the England captain has also provided seven assists for his teammates.
The former Tottenham man's total league tally of 21 contributions is the most out of any player in Europe's top five divisions.
He netted a hat-trick, his fourth of the campaign, in his previous Bundesliga outing for Bayern as they beat Augsburg 3-0 at home.
They are still yet to lose in the German top flight this season and held a six-point lead at the top of the table heading into Saturday's match.
This has increased to seven, although Eintracht Frankfurt play their game in hand against Heidenheim on Sunday.
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Dortmund last beat Bayern at home in 2018 when they won 3-2 but have claimed victory in just two matches since at all venues.
One of those came at the Allianz Arena last season to dent their opposition's title hopes heading into the crucial run-in.
They sit fifth in the table after 12 matches and are ten points behind Bayern following their second draw of the campaign.
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Bayern will be hoping Kane is fit for their next league game, which is up against Heidenheim on December 7.
But until there is any further update, it's unknown how many games, if any at all, the star will be absent for.