The Lionesses will receive a huge send-off before they travel to Australia for football's showpiece that gets underway in late July.
Sarina Wiegman's side will be focused on preparation while hoping for a victory to head into the tournament with some positive momentum.
The game takes place only four days before England fly to the other side of the world to begin their training camp down under.
England Women vs Portugal Women: talkSPORT coverage
This game will be played on Saturday, July 1.
Kick-off at Stadium MK is scheduled for 3:15pm.
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talkSPORT will provide live coverage of England's Women's World Cup send-off.
Commentary will then come from Joe Shennan and Lucy Ward.
To tune in to talkSPORT or talkSPORT 2 through the website, click HERE for the live stream.
You can also listen via the talkSPORT app, on DAB digital radio, through your smart speaker and on 1089 or 1053 AM.
England Women vs Portugal Women: Team news
Wiegman will crucially be able to assess her World Cup squad ahead of the tournament in this game.
Euro 2022 stars Beth Mead and Leah Williamson are both absent for this clash and the whole tournament after suffering ACL injuries.
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England Women's World Cup Squad
Goalkeepers: Mary Earps, Hannah Hampton, Ellie Roebuck
Defenders: Millie Bright, Lucy Bronze, Jess Carter, Niamh Charles, Alex Greenwood, Esme Morgan, Lotte Wubben-Moy
Midfielders: Laura Coombes, Jordan Nobbs, Georgia Stanway, Ella Toone, Katie Zelem, Kiera Walsh
Strikers: Rachel Daly, Beth England, Lauren Hemp, Lauren James, Katie Robinson, Chloe Kelly, Alessia Russo
England Women vs Portugal Women: What has been said?
Wiegman: "I'm really pleased to have a competitive game for our last home fixture.
"We had two big games in April against Brazil and Australia where we learned so much. This will be another challenging match against a team that have performed well and are going to their first World Cup.
"Portugal will also want to show they can be a threat to teams in Australia, and they have very technical players with good ability. For us, it will be important to come together again as a team after the end of the club season and feel the support of our fans.
"We'll do our best to give them a good performance. It will only be three weeks until our opening World Cup game so it will be such an important moment in our preparation."