The Three Lions' skipper was in pain during the second half of the 6-2 victory over Iran on Monday following a challenge from Morteza Pouraliganji.
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Despite staying on the pitch until the 75th minute, the 29-year-old was eventually replaced by Callum Wilson.
Kane was then pictured after the match with strapping around his right ankle, causing concern about the injury.
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However reports from The Athletic have said he'll now undergo a scan to decide if the injury will keep him out of England's second match in Group B.
Despite the concern, talk of him missing the USA match was played down by manager Gareth Southgate after the Iran win, with the head coach saying: "I think Harry's fine. It looked like a bad tackle but he carried on in the game.
"We took him off because we felt it was a moment in the game we could do that."
However, with England's World Cup campaign in its infancy, Southgate will be keen to avoid any risks with regards to arguably his most important player.
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In the Group B opener, Kane set up two goals in what was a convincing win.
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