The Argentine striker was playing for Jose Mourinho's side in their Serie A clash with Lecce when he stepped up to take a penalty with the scores level at 1-1.
Advertisement
Standing composed over the ball, Dybala stroked the penalty past goalkeeper Wladimiro Falcone to give Roma a 2-1 lead in the match, but immediately showed his discomfort.
Advertisement
While heading off to celebrate, the striker grimaced as his teammates joined him before they started to look concerned themselves.
With the 28-year-old clutching his thigh, Dybala hobbled off the pitch and was immediately replaced by former Manchester United midfielder Nemanja Matic.
The forward looked certain to feature for Argentina in the upcoming World Cup which gets underway next month.
But, according to Roma boss Mourinho, it seems that Dybala's hopes of being passed fit are slim.
Advertisement
Speaking to DAZN, Mourinho said: I say [Dybala is] bad, not to say very, very bad.
Advertisement
"Unfortunately, it is more very bad than [just] bad."
Advertisement
Asked if Dybala could return before the end of the year, Mourinho added: "I'm not a doctor and I haven't spoken to one, but from my experience, from what I understood from talking to Paulo, it [will be] difficult."
Bayern Munich 
FC Augsburg 
Paris Saint Germain 
Toulouse 
CSKA Moscow 
FC Rostov 
Leicester 
Chelsea 
Valencia 
Betis 
Dynamo Kyiv 
Chornomorets Odesa 
Verona 
Inter 
Bayer Leverkusen 
FC Heidenheim 
Borussia Dortmund 
SC Freiburg 
VFL Wolfsburg 
Union Berlin 
Arsenal 
Nottingham Forest 
Everton 
Brentford 
Spartak Moscow 
Lokomotiv Moscow 
Rangers 
Dundee Utd 
Atletico Madrid 
Alaves 
Lens 
Marseille 
Ingulets Petrove 
Shakhtar Donetsk 
AC Milan 
Juventus 
ST Malo 
Bordeaux 
Manchester City 
Tottenham 
Eintracht Frankfurt 
Werder Bremen 
Girona 
Espanyol 
Feyenoord 
Heerenveen 
ST Etienne 
Montpellier 
Grenoble Foot 38 
Heart Of Midlothian 
Celtic 
Reims 
Lyon 
PSV 
Groningen 
Celta Vigo 
Barcelona 
Benfica 
Estrela Da Amadora