Mikel Arteta's men bypassed the playoff stage by finishing top of Group A, seeing off the threat of PSV Eindhoven, Bodo/Glimt and FC Zurich to collect 15 points from their six games.
The Gunners enter Thursday's showdown on the back of a pulsating 3-2 win over Bournemouth in the Premier League, recovering from a two-goal deficit to remain five points clear of Manchester City at the summit.
Here, Sports Mole rounds up Arsenal's latest injury and suspension news ahead of their clash with Sporting, who defeated Midtjylland 5-1 on aggregate in the knockout round playoffs after dropping down from the Champions League.
Gabriel Jesus
Status: Out
Type of injury: Knee
Possible return date: March 19 (vs. Crystal Palace)
Gabriel Jesus is inching ever closer to a return from knee surgery and is now working with the ball at his feet in training, but it is still expected to be another couple of weeks before he can make his first-team return.
Mohamed Elneny
Status: Out
Type of injury: Knee
Possible return date: Unknown
In contrast, Mohamed Elneny's knee operation is likely to keep him out for the remainder of the season, but he has nevertheless been rewarded with a new one-year contract at the Emirates.
Eddie Nketiah
Status: Major doubt
Type of injury: Ankle
Possible return date: March 9 (vs. Sporting)
Eddie Nketiah has been dealing with a knock to the ankle for the past couple of weeks and was not available for the clash with Bournemouth, but the striker should be in with a chance of making the trip to Portugal.
Leandro Trossard
Status: Out
Type of injury: Groin
Possible return date: Unknown
January signing Leandro Trossard, who has made an immediate impact in the first team, was withdrawn in the first half against Bournemouth due to a groin problem, although it is still unclear how severe the Belgian's problem is.
ARSENAL'S SUSPENSION LIST
Arsenal have no players suspended for this match, but left-back Kieran Tierney will miss the second leg if he picks up a booking here.
CSKA Moscow 
FC Rostov 
Leicester 
Chelsea 
Valencia 
Betis 
Dynamo Kyiv 
Chornomorets Odesa 
Verona 
Inter 
Bayer Leverkusen 
FC Heidenheim 
Borussia Dortmund 
SC Freiburg 
VFL Wolfsburg 
Union Berlin 
Arsenal 
Nottingham Forest 
Everton 
Brentford 
Spartak Moscow 
Lokomotiv Moscow 
Rangers 
Dundee Utd 
Atletico Madrid 
Alaves 
Lens 
Marseille 
Ingulets Petrove 
Shakhtar Donetsk 
AC Milan 
Juventus 
ST Malo 
Bordeaux 
Manchester City 
Tottenham 
Eintracht Frankfurt 
Werder Bremen 
Girona 
Espanyol 
Feyenoord 
Heerenveen 
ST Etienne 
Montpellier 
Grenoble Foot 38 
Heart Of Midlothian 
Celtic 
Reims 
Lyon 
PSV 
Groningen 
Celta Vigo 
Barcelona 
Benfica 
Estrela Da Amadora 
Sparta Rotterdam 
AZ Alkmaar 
Southampton 
Liverpool