Los Blancos - who have not got their hands on the crown since the 2013-14 season - have seen off Cacereno, Villarreal and Atletico Madrid to progress to the final four, and they renewed hostilities with the latter in the weekend's La Liga clash.
An equaliser from teenage starlet Alvaro Rodriguez saw Carlo Ancelotti's side come away with a point in a 1-1 draw in the Madrid derby, and the Spanish champions host Barcelona this week before the second leg at Camp Nou on April 5.
Here, Sports Mole rounds up Real Madrid's latest injury and suspension news ahead of Thursday's first leg with Barcelona, who saw off Real Sociedad 1-0 in the last eight.
David Alaba
Status: Out
Type of injury: Hamstring
Possible return date: March 19 (vs. Barcelona)
David Alaba sustained a hamstring injury in the first leg of Real's Champions League last-16 tie versus Liverpool, and he is expected to remain out for between three and four weeks, potentially putting him in contention to return for next month's Clasico in La Liga.
Rodrygo
Status: Out
Type of injury: Glute
Possible return date: March 5 (vs. Real Betis)
Rodrygo was another casualty of the 5-2 win at Anfield with a glute problem, which is not as serious as Alaba's injury, but the Brazilian will likely miss out here as he eyes a possible return for the weekend's clash with Real Betis.
Ferland Mendy
Status: Out
Type of injury: Thigh
Possible return date: April 2 (vs. Real Valladolid)
Ferland Mendy sustained a thigh injury during Real's Copa del Rey quarter-final win over Atletico in January, and the left-back may not be seen in a Real Madrid shirt until after the March international break.
REAL MADRID'S SUSPENSION LIST
Luka Modric picked up his fifth yellow card of the season in the weekend's Madrid derby, but he will serve that suspension in the weekend's La Liga clash with Real Betis, meaning that Real Madrid have no players banned for this match.