The two teams are at complete opposite ends of the table and require points for drastically different reasons in what is a crucial game for each.
Match preview
Oxford head into this weekend clinging onto a place in the playoffs with just one point separating them and Stevenage, placing plenty of pressure on the team to perform.
Des Buckingham's men will need to show a drastic improvement in their recent form to ensure they do not slip out of the top six, as they have failed to win in their previous three matches.
The best Oxford could mount on Tuesday was a 2-2 draw against Portsmouth, with James Henry scoring a last-minute equaliser to ensure they even left that game with a point.
The last time the U's secured a win was against another team in the relegation zone, Carlisle United, which might provide some confidence as they prepare to face Reading.
However, the Royals did manage to hold Oxford to a 1-1 draw when they faced each other in December, even though Buckingham's men dominated the game with 61% of the possession.
Reading have faced an uphill battle from the start of the season due to the four-point deduction they were handed, and since then they have been fighting to try and secure survival in the division.
Heading into this weekend, a victory could be enough to take the Royals out of the bottom four, depending on the result of the Port Vale match.
That will provide plenty of incentive for the team, who should have had an injection of confidence after going two games in a row without a defeat heading into this fixture.
Ruben Selles saw his team follow a 1-0 victory over Derby County with a 1-1 draw against Leyton Orient in midweek, proving that Reading have no problem taking points from teams in the top half.
However, only one of Reading's eight wins this season has taken place on the road which is a cause for concern and an area that the team must address in the second half of the campaign.
Oxford United League One form:
L
W
W
L
L
D
Oxford United form (all competitions):
L
L
W
L
L
D
Reading League One form:
D
W
D
L
W
D
Reading form (all competitions):
W
L
D
L
W
D
Team News
There is still concern for Sam Long and Joe Bennett heading into this weekend for the U's, with both men having been dealing with calf problems recently.
Jamie Cumming and Fin Stevens were added to the injury list in midweek, but both men are fighting to be fit for this Saturday, and are in contention.
The weekend comes too soon for Billy Bodin, Will Goodwin and Josh Murphy, all of whom are still on the sidelines as Buckingham believes they will be back to face Blackpool.
Reading will no longer be able to call upon Tom McIntyre, who made the move to Portsmouth this week, while Taylan Harris joined Luton Town.
Oxford United possible starting lineup:
Eastwood; Thorniley, Moore, Brown, Leigh; McGuane, Brannagan; Goodrham, Rodrigues, Burey; Harris
Reading possible starting lineup:
Button; Yiadom, Bindon, Holmes, Dorsett; Azeez, Wing, Savage, Knibbs, Mukairu; Smith
We say: Oxford United 0-1 Reading
Oxford United will be the favourites walking into this weekend due to the league positions of each club, but recent form tells a different story.
Reading have been grinding out results against some of League One's strongest teams lately, and Saturday could provide another upset.