The hosts edged out Rosengard in their opening group match, while the Catalan giants began the defence of their trophy with a comfortable 5-0 win over Benfica.
Match preview
The hosts are competing under the Eintracht Frankfurt banner for the first time in the Champions League main draw after negotiating qualifying ties against Slovacko, Juventus and Sparta Prague.
Prior to their merger with the men's club in 2020, they enjoyed significant European success as FFC Frankfurt, winning four Champions League trophies, including their most recent triumph in the 2014-15 campaign when they beat Paris Saint-Germain in the final.
While they may technically be the second most successful club in the competition, they are now looking to create their own European history as Eintracht Frankfurt.
They certainly made a positive start to their group stage campaign after goals from Tanja Pawollek and Barbara Dunst guided them to a 2-0 away victory over Swedish outfit Rosengard.
The German side followed that result with another away win in Saturday's league clash with Nurnberg, which helped extend their unbeaten run to nine competitive games - a streak that will come under threat when they face the defending European champions on Wednesday.
Barcelona will travel to Germany in scintillating form, having won all 10 competitive matches this season, including their last two against Benfica and Real Madrid in the Champions League and Liga F respectively.
The Catalan side cruised to a dominant 5-0 victory over the Portuguese champions in their opening group match, as Alexia Putellas and Aitana Bonmati both netted braces before Asisat Oshoala scored her sixth goal of the season.
The Spanish champions went on to register five more goals without reply in Sunday's Clasico, with Bonmati, Caroline Graham Hansen, Mariona Caldentey, Claudia Pina and Vicky Lopez all getting on the scoresheet at Estadi Olímpic Lluís Companys.
Having racked up 30 goals across their last five competitive matches, Barcelona will be targeting another dominant performance in Wednesday's away clash.
However, they will be mindful of the fact their most recent Champions League defeat took place on their last trip to Germany in December 2022 when they were beaten 3-1 by Bayern at the Allianz Arena.
Eintracht Frankfurt Women Women's Champions League form:
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Eintracht Frankfurt Women form (all competitions):
Barcelona Women Women's Champions League form:
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Barcelona Women form (all competitions):
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Team News
Niko Arnautis made a number of changes to his Frankfurt side at the weekend, but he is expected to revert back to the lineup that started against Rosengard.
Sophia Kleinherne will feature in central defence alongside Sara Doorsoun-Khajeh, while Pia-Sophie Wolter and Verena Hanshaw are set to operate as the two full-backs.
Meanwhile, Laura Freigang will lead the line for the hosts, and the Germany international is expected to be flanked by Nicole Anyomi and Lara Prasnikar.
As for the visitors, they are expected to be without the services of Jana Fernandez, Bruna Vilamala and Fridolina Rolfo.
There are also doubts over the availability of Putellas and Irene Paredes, who are struggling with illness and injury respectively.
After seeing his side breeze past Real Madrid, Giraldez could be tempted to name an unchanged lineup for Wednesday's encounter.
Eintracht Frankfurt Women possible starting lineup:
Johannes; Wolter, Kleinherne, Doorsoun-Khajeh, Hanshaw; Reuteler, Pawollek, Dunst; Anyomi, Prasnikar, Freigang
Barcelona Women possible starting lineup:
Coll; Bronze, Engen, Leon, Batlle; Bonmati, Walsh, Guijarro; Hansen, Paralluelo