The Dutch have looked back to their old best so far this summer, winning three from three in Group C and scoring the most goals in the group stage.
They now take on a Czech side who finished third in Group D and were beaten by England last time out.
The winner of this tie in Budapest will face either Wales or Denmark in the quarter-finals.
Netherlands v Czech Republic: How to listen
This last 16 tie will take place at 5pm on Sunday, June 27.
Dan Windle will be your host and commentary will come from Adam Bridge and Trevor Sinclair.
To tune in, click the radio player below or HERE for the live stream.
Here are the other ways to listen…
App iPhone - Download from the Apple store
Android - Download from Google Play
Radio - talkSPORT is available across the United Kingdom via DAB digital radio and on 1089 or 1053 AM.
TV - Listen to talkSPORT through your TV on the following channels: Sky: Channel 0108 Virgin Media: Channel 927 Freeview: Channel 723 Freesat: Channel 731
Euro 2020 fixtures
Wales vs Denmark (5pm, Saturday June 26, Amsterdam)
Italy vs Austria (8pm, Saturday June 26, London)
Netherlands vs Czech Republic (5pm, Sunday June 27, Budapest)
Belgium vs Portugal (8pm, Sunday June 27, Seville)
Croatia vs Spain (5pm, Monday June 28, Copenhagen)
France vs Switzerland (8pm, Monday June 28, Bucharest)
England vs Germany (5pm, Tuesday June 29, London)
Sweden vs Ukraine (8pm, Tuesday June 29, Glasgow)
Netherlands v Czech Republic: Squads
Netherlands
Goalkeepers: Marco Bizot (AZ), Tim Krul (Norwich), Maarten Stekelenburg (Ajax)
Defenders: Nathan Aké (Manchester City), Daley Blind (Ajax), Matthijs de Ligt (Juventus), Stefan de Vrij (Inter Milan), Denzel Dumfries (PSV), Patrick van Aanholt (Crystal Palace), Joël Veltman (Brighton), Owen Wijndal (AZ)
Midfielders: Frenkie de Jong (Barcelona), Marten de Roon (Atalanta), Ryan Gravenberch (Ajax), Davy Klaassen (Ajax), Teun Koopmeiners (AZ), Quincy Promes (Spartak Moskva), Jurriën Timber (Ajax), Georginio Wijnaldum (Liverpool)
Forwards: Steven Berghuis (Feyenoord), Luuk de Jong (Sevilla), Memphis Depay (Lyon), Cody Gakpo (PSV), Donyell Malen (PSV Eindhoven), Wout Weghorst (Wolfsburg)
Czech Republic
Goalkeepers: Tomáš Koubek (Augsburg), Aleš Mandous (Olomouc), Tomáš Vaclík (Sevilla)
Defenders: Jan Bořil (Slavia Praha), Jakub Brabec (Viktoria Plzeň), Ondřej Čelůstka (Sparta Praha), Vladimír Coufal (West Ham), Pavel Kadeřábek (Hoffenheim), Tomáš Kalas (Bristol City), Tomáš Holeš (Slavia Praha), Aleš Matějů (Brescia), David Zima (Slavia Praha)
Midfielders: Antonín Barák (Verona), Vladimír Darida (Hertha Berlin), Jakub Jankto (Sampdoria), Alex Král (Spartak Moskva), Lukáš Masopust (Slavia Praha), Jakub Pešek (Sparta Praha), Michal Sadílek (Liberec), Petr Ševčík (Slavia Praha), Tomáš Souček (West Ham)
Goalkeepers: Tomáš Koubek (Augsburg), Aleš Mandous (Olomouc), Tomáš Vaclík (Sevilla)
Defenders: Jan Bořil (Slavia Praha), Jakub Brabec (Viktoria Plzeň), Ondřej Čelůstka (Sparta Praha), Vladimír Coufal (West Ham), Pavel Kadeřábek (Hoffenheim), Tomáš Kalas (Bristol City), Tomáš Holeš (Slavia Praha), Aleš Matějů (Brescia), David Zima (Slavia Praha)
Midfielders: Antonín Barák (Verona), Vladimír Darida (Hertha Berlin), Jakub Jankto (Sampdoria), Alex Král (Spartak Moskva), Lukáš Masopust (Slavia Praha), Jakub Pešek (Sparta Praha), Michal Sadílek (Liberec), Petr Ševčík (Slavia Praha), Tomáš Souček (West Ham)
Forwards: Adam Hložek (Sparta Praha), Michael Krmenčík (PAOK), Tomáš Pekhart (Legia), Patrik Schick (Leverkusen), Matěj Vydra (Burnley)