Los Blancos were back in action after the World Cup against Real Valladolid on Friday, recording a 2-0 victory courtesy of a late double from Karim Benzema.
Real Madrid are entering a busy run of matches, which will include a Spanish Super Cup semi-final with Valencia, so Ancelotti will ring the changes for this game with fourth-tier Cacereno.
Dani Ceballos and Marco Asensio could keep their spots from the Real Valladolid game, but it might otherwise be a new group of players for this contest.
Andriy Lunin is set to start between the sticks, while Lucas Vazquez, Nacho, Jesus Vallejo and Alvaro Odriozola could come into the back four.
Eduardo Camavinga and Aurelien Tchouameni were only substitutes on Friday due to their involvement in the World Cup final, but the pair could be brought in for starts here.
There will also be alterations in the final third of the field, with Rodrygo and Eden Hazard potentially coming in for Benzema and Vinicius Junior, who are set to be rested.
Ancelotti is also likely to name some Castilla players in his squad for the Copa del Rey contest.
Real Madrid possible starting lineup: Lunin; Vazquez, Nacho, Vallejo, Odriozola; Camavinga, Tchouameni, Ceballos; Asensio, Rodrygo, Hazard