Jamal Musiala is the biggest absence from the squad, with the Bayern Munich attacker, who has four goals and three assists at club level this term, unavailable for selection due to a hamstring problem.
Borussia Dortmund's Nico Schlotterbeck has been overlooked, while Felix Nmecha has been forced to withdraw from the squad due to a hip issue.
Robin Gosens is also absent for personal reasons, with David Raum earning a late call-up, while there are five uncapped players in the latest squad, including outfield trio Marvin Ducksch, Robert Andrich and Grischa Promel.
Germany, who are Euro 2024 hosts, will play again on Tuesday against Austria, with Nagelsmann set to hand a number of players opportunities to impress over the two games.
A strong XI is set to take to the field for the first whistle here, though, with Leroy Sane and Florian Wirtz likely to operate in the wide areas, while Musiala's absence could see Julian Brandt brought in.
Niclas Fullkrug has nine goals in 11 caps for his country and is again set to be given the nod in the final third of the field, with Thomas Muller, Serge Gnabry and Kai Havertz likely to be on the bench.
Germany possible starting lineup: Ter Stegen; Tah, Hummels, Rudiger, Henrichs; Kimmich, Gundogan; Wirtz, Brandt, Sane; Fullkrug