The Manchester City man is in the final stages of his recovery from groin surgery and has been training with the team, but by his own admission, he is unlikely to be risked here.
Leicester City playmaker James Maddison has also missed a couple of days' worth of training, but it would have been a surprise to see him line up from the start here.
Aaron Ramsdale and Nick Pope lead the way at the top of the Premier League clean sheet charts, but Jordan Pickford should start the World Cup as England's number one, most likely with Harry Maguire and John Stones protecting him.
A four-man backline would likely see Kieran Trippier and Luke Shaw occupy the wide areas, and Southgate is facing a dilemma over Declan Rice's midfield partner.
Kalvin Phillips was an integral cog at Euro 2020, but his shoulder injury and subsequent lack of minutes could see Southgate opt to bring in Jude Bellingham instead.
Raheem Sterling, Mason Mount and Phil Foden are seemingly nailed on to occupy spots in the final third behind Harry Kane, who needs just three more goals to overtake Ellen White and Wayne Rooney and become England's highest goalscorer of all time.
England possible starting lineup: Pickford; Trippier, Stones, Maguire, Shaw; Rice, Bellingham; Sterling, Mount, Foden; Kane
AC Milan 
Juventus 
ST Malo 
Bordeaux 
Manchester City 
Tottenham 
Eintracht Frankfurt 
Werder Bremen 
Girona 
Espanyol 
Feyenoord 
Heerenveen 
ST Etienne 
Montpellier 
Grenoble Foot 38 
Heart Of Midlothian 
Celtic 
Reims 
Lyon 
PSV 
Groningen 
Celta Vigo 
Barcelona 
Benfica 
Estrela Da Amadora 
Sparta Rotterdam 
AZ Alkmaar 
Southampton 
Liverpool 
Como 
Fiorentina 
Sevilla 
Rayo Vallecano 
Ajax 
Pec Zwolle 
Palermo 
Sampdoria 
Ipswich 
Manchester Utd 
Napoli 
Roma 
Moreirense 
FC Porto 
Leganes 
Real Madrid 
Lazio 
Bologna 
Empoli 
Udinese 
CSKA Moscow 
Rubin 
Slovan Bratislava 
Sparta Praha 
Atletico Madrid 
Valencia 
Stade Brestois 29