Los Blancos saw their nine-game winning run in all competitions come to an end on Sunday when they were held to a 1-1 draw by Osasuna in La Liga.
Carlo Ancelotti's side have been victorious in their opening two Champions League games this term, beating Celtic 3-0 on September 6 before recording a 2-0 win over RB Leipzig one week later.
Here, Sports Mole rounds up Real Madrid's latest injury and suspension news ahead of their contest with Shakhtar, who have picked up four points from their first two European games to sit second in Group F.
Lucas Vazquez
Status: Out
Type of injury: Thigh
Possible return date: October 8 (vs. Getafe)
Lucas Vazquez is still out with the thigh problem that he picked up against Mallorca in the league last month, but the Spaniard could be available for selection against Getafe.
Luka Modric
Status: Minor doubt
Type of injury: Hip
Possible return date: October 5 (vs. Shakhtar)
Luka Modric was back in training for Real Madrid on Monday after seemingly overcoming a hip problem, so he could make his return in the European clash with Shakhtar.
Thibaut Courtois
Status: Major doubt
Type of injury: Back
Possible return date: October 5 (vs. Shakhtar)
Thibaut Courtois was unavailable for selection against Osasuna due to a back problem, and the Belgium international could also miss out for the Spanish and European champions here, having been unable to train with his teammates on Monday.
REAL MADRID'S SUSPENSION LIST
Real Madrid have no players suspended for the clash with Shakhtar.