The North London derby isn't the only WSL fixture attracting huge crowds, though, with matches across the women's games seeing a surge in ticket sales following a summer of success for the Lionesses.
Over 50,000 people are expected to be in attendance to watch the likes of European champions Leah Williams and Beth Mead in the derby on Saturday - which you can follow with LIVE commentary on talkSPORT.
The game is set to better the previous record set in the reverse fixture at the Tottenham Hotspur Stadium in 2019, which attracted a crowd of 38,262.
The WSL season is just one game in after the opening weekend was delayed due to the passing of the late Queen Elizabeth II.
But whilst there isn't much riding on Saturday's game other than bragging rights, tickets were snapped up in no time, with England's Women's Euro 2022 victory causing a increase in appetite for women's football.
Arsenal Women's home ground, National League Boreham Wood's Meadow Park, holds a capacity of 4,500, and season tickets sold out at the beginning of August.
This weekend's fixture will see the Gunners play in front of over ten times their normal home crowd.
"You can really feel the excitement around the game at the moment so it's amazing to see that translate into a record-breaking attendance," Arsenal head coach Jonas Eidevall said.
"Now it's our job to repay that enthusiasm with a big performance in this crucial clash for our supporters."
Arsenal are set to play two further games at the Emirates, as well as using the stadium for their Champions League group stage matches should they beat Ajax in their final qualifying match.
Tottenham's fan base has also seen a sharp rise in numbers this term, with their season tickets at new home ground, Leyton Orient's Brisbane Road, also selling out this week.
Arsenal aren't the only women's team playing at their club's men's stadiums, either, with sides across the women's game using the big grounds for a few of their fixtures.
WSL champions Chelsea were set to play at Stamford Bridge on the opening day against London rivals West Ham in front of a bumper crowd, although that match was postponed due to the passing of the late Queen.
Should this weekend's attendance break the WSL record, it will add to the growing list of feats smashed by women's football this year, with the crowd at England's win over Germany at Wembley of 87,192 breaking the record of any European Championship match.
Tune into talkSPORT for LIVE commentary of Arsenal vs Tottenham in the Women's Super League, Saturday September 24, kick off: 1:30pm