Both United and City will look to secure the local bragging rights when they each make the short trip to Leigh Sports Village for the sold-out fixture.
Marc Skinner's Red Devils bounced back from their 6-1 defeat against reigning champions Chelsea with a 2-0 win over Birmingham on Sunday.
Meanwhile, Gareth Taylor's City will hope to use the derby to kickstart their floundering start to the new season.
The Sky Blues are already six points behind neighbours United after suffering three defeats in their opening four league games so far.
Man United Women v Man City Women: talkSPORT commentary
The Women's Super League clash will take place on Saturday, October 9 with a kick-off time of 1:30pm.
Commentary will then come from Ian Danter and former Leeds forward Lucy Ward.
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Man United Women v Man City Women: Team news
Alessia Russo's introduction for Kirsty Hanson was United's only change for the 2-0 win over Birmingham on Sunday.
Defender Millie Turner remains absent following the knee injury she sustained last month in a 3-1 victory on the road at Leicester.
Martha Thomas and Kirsty Smith both missed last weekend's fixture and it yet to be confirmed whether either will be back to face City.
The visitors meanwhile have been plagued by injuries, with England trio Steph Houghton, Karen Bardsley and Lucy Bronze all sidelined.
Esme Morgan is nursing a broken leg, while Chloe Kelly, Demi Stokes, Hayley Raso and Ellie Roebuck are also in the treatment room.
Man United Women v Man City Women: What has been said?
Leah Galton has lifted the lid on United's positive start to the season that has seen them sit fourth in the table after three wins from four games.
"It has [been a good start]," Galton told the club's website.
"I always think it can be better obviously. We're working hard in training and a lot has changed because obviously we have a new coach now, so we're adapting to things but nine points out of 12, I'd definitely take that."
Houghton: "The Manchester Derby means absolutely everything.
"Since United came into the league and in football in general, it's the most special fixture to look out for in the calendar.
"There's always added spice when you play your local rivals and they haven't beaten us so far in the league, which is something we're proud of and want to continue.
"For us as a team, we're capable of winning any football game and our mentality is to win as many games as we possibly can.
"It's certainly one for us to look forward to and we'll hopefully, keep Manchester Blue!"