Xavi's side were stunned 1-0 by Almeria on Sunday night courtesy of El Bilal Toure's winner, but Los Blancos' 1-1 draw with city rivals Atletico Madrid means that they still boast a healthy seven-point lead at the top of the table.
La Blaugrana edged past Real Sociedad 1-0 in the Copa del Rey quarter-finals thanks to Ousmane Dembele's effort, and they travel to the Bernabeu this week before hosting Real Madrid in the second leg at Camp Nou on April 5.
Here, Sports Mole rounds up Barcelona's latest injury and suspension news ahead of the first leg with Real Madrid, who overcame Atletico in extra time in the quarter-finals.
Ansu Fati
Status: Minor doubt
Type of injury: Knee
Possible return date: March 2 (vs. Real Madrid)
Ansu Fati was unavailable for the weekend's defeat to Almeria after sustaining a bruised knee in training, but Xavi is confident that the Spanish attacker will be fit for Thursday's game.
Ousmane Dembele
Status: Out
Type of injury: Thigh
Possible return date: March 19 (vs. Real Madrid)
Ousmane Dembele is making good progress in his recovery from a thigh injury, which he suffered at the end of January, and the Frenchman could potentially return for another El Clasico with Real Madrid on March 19.
Pedri
Status: Out
Type of injury: Hamstring
Possible return date: March 12 (vs. Athletic Bilbao)
Pedri was a first-half casualty of Barcelona's Europa League clash with Manchester United at Camp Nou two Thursdays ago with a thigh problem, but the issue is not too severe, and there is reportedly hope that he could return for the meeting with Athletic Bilbao on March 12.
BARCELONA'S SUSPENSION LIST
Barcelona have no players suspended for the first leg, but Gavi and Raphinha will miss the weekend's La Liga clash with Valencia due to yellow card accumulation.